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Fig. 4 | Journal of Orthopaedic Surgery and Research

Fig. 4

From: FGD5-AS1 facilitates the osteogenic differentiation of human bone marrow-derived mesenchymal stem cells via targeting the miR-506-3p/BMP7 axis

Fig. 4

Decreased miR-506-3p rescues the suppressive effect of FGD5-AS1 depletion on the osteogenesis of hBMSCs. A RT-qPCR analysis showed the miR-506-3p levels in hBMSCs transfected with NC inhibitor and miR-506-3p inhibitor. B RT-qPCR analysis showed the levels of osteogenic markers in hBMSCs transfected with shNC, shFGD5-AS1, and shFGD5-AS1+miR-506-3p inhibitor. C, D ALP staining and ALP activity assays showed the ALP activity of hBMSCs transfected with shNC, shFGD5-AS1, and shFGD5-AS1+miR-506-3p inhibitor. E Alizarin Red staining showed the cell mineralization ability of hBMSCs transfected with shNC, shFGD5-AS1, and shFGD5-AS1+miR-506-3p inhibitor. F CCK-8 assay determined the viability of hBMSCs transfected with shNC, shFGD5-AS1, and shFGD5-AS1+miR-506-3p inhibitor. *P < 0.05

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Journal of Orthopaedic Surgery and Research

ISSN: 1749-799X