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Fig. 2 | Journal of Orthopaedic Surgery and Research

Fig. 2

From: FGD5-AS1 facilitates the osteogenic differentiation of human bone marrow-derived mesenchymal stem cells via targeting the miR-506-3p/BMP7 axis

Fig. 2

Downregulated FGD5-AS1 suppresses the osteogenesis of hBMSCs. A RT-qPCR was used to determine the FGD5-AS1 expression in hBMSCs transfected with shFGD5-AS1 or shNC. B, C RT-qPCR and western blotting assays showed the protein levels of osteogenic markers in hBMSCs transfected with shFGD5-AS1 or shNC. D, E ALP activity and ALP staining assays exhibited the ALP activity of hBMSCs transfected with shFGD5-AS1 or shNC. F Alizarin Red staining showed the cell mineralization ability of hBMSCs transfected with shFGD5-AS1 or shNC. G CCK-8 assay showed the viability of hBMSCs transfected with shFGD5-AS1 or shNC. *P < 0.05

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Journal of Orthopaedic Surgery and Research

ISSN: 1749-799X