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Fig. 1 | Journal of Orthopaedic Surgery and Research

Fig. 1

From: FGD5-AS1 facilitates the osteogenic differentiation of human bone marrow-derived mesenchymal stem cells via targeting the miR-506-3p/BMP7 axis

Fig. 1

Levels of FGD5-AS1, miR-506-3p, and BMP7 after osteogenic differentiation of BMSCs. A RT-qPCR was used to detect the expression of FGD5-AS1 in bone tissues from patients with osteoporosis and healthy individuals. B–H RT-qPCR analysis was used to detect the expression of FGD5-AS1, miR-506-3p, BMP7, and osteogenesis-related genes (RUNX2, OCN, OSX, and OPN) in hBMSCs treated with OM for 14 days. I Western blotting assay showed the protein levels of osteogenesis-related genes in hBMSCs treated with osteogenic medium (OM) for 14 days. J, K ALP activity and ALP staining assays revealed the ALP activity of hBMSCs treated with OM for 14 days. K Alizarin Red staining showed the cell mineralization ability of hBMSCs treated with OM for 14 days. M CCK-8 assay showed the viability of hBMSCs during the process of osteogenic differentiation. *P < 0.05

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