Table 1 Primers sequences with their corresponding RT-PCR product size
From: Iron accumulation deteriorated bone loss in estrogen-deficient rats
Gene primer | Sequence | Size (bp) |
|---|---|---|
GAPDH | Forward:5′-GACATGCCGCCTGGAGAAAC-3′ Reverse:5′-AGCCCAGGATGCCCTTTAGT-3′ | 128 |
Hepcidin | Forward:5′-ACAGAAGGCAAGATGGCACT-3′ Reverse:5′-GAAGTTGGTGTCTCGCTTCC-3′ | 201 |
FPN | Forward:5′-TACGGAAACAGCCTCCTCTT-3′ Reverse:5′-GCATTCTTATCCACCCAGTCA-3′ | 101 |
DMT-1 | Forward:5′-AGCCATCAGAGCCAGTGTGT-3′ Reverse:5′-CCCCAGTGTTTCCCAACTAA-3′ | 111 |
DCYTB | Forward:5′-TCTCTTCCAAAGTCAGCCCTAC-3′ Reverse:5′-CTCAGTCAACACAATCGCTCTC-3′ | 107 |