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Fig. 2 | Journal of Orthopaedic Surgery and Research

Fig. 2

From: 4-Methylumbelliferone suppresses catabolic activation in anterior cruciate ligament-derived cells via a mechanism independent of hyaluronan inhibition

Fig. 2

Matrix metalloproteinase (MMP)-1, MMP-3, and interleukin (IL)-6 immunohistochemistry in the anterior cruciate ligament (ACL) explants. ACL explants were cultured in a medium with or without 2.0 ng/mL IL-1β in the presence or absence of 1.0 mM 4-MU for 7 days. Here, we show the representative images of MMP-1, MMP-3, and IL-6 staining of the ACL explants. Scale bars represent 20 μm. Staining for MMP-1, MMP-3, and IL-6 indicated the highest protein expression in the 2.0 ng/mL IL-1β-treated explants (white arrows). The expression in explants that were co-treated with 2.0 ng/mL IL-1β and 1.0 mM 4-MU was substantially reduced (black arrows). (B) Statistical quantification for staining positivity of each antibody (n = 3). The values are presented as mean ± SD. *p < 0.05; **p < 0.01

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