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Fig. 5 | Journal of Orthopaedic Surgery and Research

Fig. 5

From: Hsa_circ_0004674 promotes osteosarcoma doxorubicin resistance by regulating the miR-342-3p/FBN1 axis

Fig. 5

FBN1 was targeted by miR-342-3p. A The binding sites between miR-342-3p and FBN1 3’UTR was predicted by Starbase software. The interaction between miR-342-3p and FBN1 was detected by dual-luciferase reporter assay (B) and RNA pull-down assay (C). D-E FBN1 mRNA and protein expression levels in DXR-resistant OS tissues and DXR-sensitive OS tissues were measured by qRT-PCR and WB analysis. F The FBN1 protein expression in DXR-resistant OS cells (U2OS_R and KHOS_R) and normal OS cells (U2OS and KHOS) was detected by WB analysis. G The correlation between miR-342-3p and FBN1 in OS tissues was analyzed by Pearson’s correlation coefficient. *P < 0.05

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