Fig. 2
From: Hsa_circ_0004674 promotes osteosarcoma doxorubicin resistance by regulating the miR-342-3p/FBN1 axis
Hsa_circ_0004674 knockdown reduced OS DXR resistance. A The transfection efficiencies of three siRNAs for hsa_circ_0004674 were evaluated by detecting hsa_circ_0004674 expression using qRT-PCR in U2OS_R and KHOS_R cells. B-F U2OS_R and KHOS_R cells were transfected with si-NC, si-hsa_circ_0004674#1 or si-hsa_circ_0004674#2. B CCK8 assay was used to test the cell DXR resistance. Colony formation assay (C) and flow cytometry (D-E) were performed to determine the number of colonies, cell cycle process and apoptosis rate. F The p-gp and MCL-1 protein levels were tested by WB analysis. *P < 0.05