Fig. 3

Circ_0025908 acted as a sponge for miR-137. (A) The targeted miRNAs of circ_0025908 were forecast by Starbase. (B) The expression of miR-137 in RA tissues (n = 40) and normal tissues (n = 40) was detected by qRT-PCR. (C) Pearson’s correlation analysis confirmed that circ_0025908 was negatively associated with miR-137 (R = −0.627) in RA tissues. (D) QRT-PCR was used to detect the miR-137 expression. (E) Dual-luciferase reporter assay was used to verify the relationship between circ_0025908 and miR-137. miR-NC: miR-137 negative control, miR-137: miR-4319 mimic, circ_0025908-WT: circ_0025908 wild type, circ_0025908-MUT: circ_0025908 mutant type. (F) RIP assay was used to verify the relationship between circ_0025908 and miR-137. (G) RNA-pulldown assay was used to verify the relationship between circ_0025908 and miR-137. (H) QRT-PCR was used to detect the miR-137 expression. (I) The expression of miR-137 in RA cells was detected by qRT-PCR. *P < 0.05