Table 1 The sequences of all primers used in qRT-PCR
From: MiR-330-5p inhibits intervertebral disk degeneration via targeting CILP
Gene name | Primer sequence (5′-3′) |
|---|---|
CILP | Forward: GCAAAAGCATCCTGAAGATCAC |
Reverse: GGAGTCTCTGCCCTCACAAAC | |
ACAN | Forward: ACCAGACTGTCAGATACCCC |
Reverse: CATAAAAGACCTCACCCTCC | |
COL1A1 | Forward: TGACCTCAAGATGTGCCACT |
Reverse: ACCAGACATGCCTCTTGTCC3 | |
COL2A1 | Forward: ATTGCCTATCTGGACGAAGC |
Reverse: GCAGTGTACGTGAACCTGCT | |
MMP-3 | Forward: GCATTGGCTGAGTGAAAGAGAC |
Reverse: ATGATGAACGATGGACAGATGA | |
MMP-9 | Forward: GGGACGCAGACATCGTCATC |
Reverse: GGGACCACAACTCGTCATCG | |
miR-330-5p | Forward: TCTCTGGGCCTGTGTCTTAG |
Reverse: CAGTGCGTGTCGTGGAGT | |
β-actin | Forward: GTGGGGCGCCCCAGGCACCA |
Reverse: CTTCCTTAATGTCACGCACGATTTC | |
U6 | Forward: GTGCTCGCTTCGGCAGCACATATAC |
Reverse: AAAAATATGGAACGCTTCACGAATTTG |