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Fig. 4 | Journal of Orthopaedic Surgery and Research

Fig. 4

From: MiR-330-5p inhibits intervertebral disk degeneration via targeting CILP

Fig. 4

MiR-330-5p binds to CILP. (A) Bioinformatics software (ENCORI) predicted that hsa-miR-330-5p had the binding sites of the CILP 3′-untranslated region (UTR) region. (B) A dual-luciferase reporter gene analysis was conducted to test CILP relative luciferase activity. We transfected miR-330-5p mimic or NC mimic into NP cells. (C) qRT-PCR was conducted to quantify CILP expression. (D) Western blot was applied to quantify CILP protein level. (E-F) qRT-PCR was applied to measure miR-330-5p expressions in the degenerated NP tissues and cells. *P < 0.05 vs. control. **P < 0.01 vs. NC mimic. ***P < 0.001 vs. control. NC, negative control of three independent experiments

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