Fig. 3
From: MiR-330-5p inhibits intervertebral disk degeneration via targeting CILP
CILP affects the senescence and ECM production of NP cells. After the NP cells were mixed with 20 ng/ml TNF-α for approximately 24 h, the cells were transfected with si-CILP. (A) β-galactosidase staining was applied to assess NP cell senescence. (B) qRT-PCR was applied to quantify aggrecan (ACAN), collagen type I (COL1A1), collagen type II (COL2A1), MMP-3, and MMP-9 expressions in NP cells. **P < 0.01, ***P < 0.001 vs. control. #P < 0.05, ##P < 0.01 vs. TNF-α of three independent experiments