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Fig. 2 | Journal of Orthopaedic Surgery and Research

Fig. 2

From: MiR-330-5p inhibits intervertebral disk degeneration via targeting CILP

Fig. 2

Influence of CILP in the cell cycle, activity, and apoptosis of NP cells. After the NP cells were mixed with 20 ng/ml TNF-α for approximately 24 h, the cells were transfected with si-CILP. (A) Flow cytometry was applied to test the cell cycle distribution of NP cells. (B) 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was applied to test NP cell activity at 0, 24, 48, and 72 h. (C) Flow cytometry was applied to analyze NP cell apoptosis. **P < 0.01 vs. control. #P < 0.05, ##P < 0.01 vs. TNF-α of three independent experiments

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