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Fig. 2 | Journal of Orthopaedic Surgery and Research

Fig. 2

From: CircMAPK9 promotes the progression of fibroblast-like synoviocytes in rheumatoid arthritis via the miR-140-3p/PPM1A axis

Fig. 2

The influence of circMAPK9 on cell proliferation, apoptosis, cycle distribution, migration, invasion, and inflammatory response in RA-FLSs. A Knockdown efficiency of circMAPK9 was detected by qRT-PCR in RA-FLSs with transfection of si-NC, si-circMAPK9#1, si-circMAPK9#2, or si-circMAPK9#3. B–J RA-FLSs were transfected with si-NC or si-circMAPK9#2. B Cell proliferation was assessed by MTT assay. C, D Cell apoptosis and cycle distribution were measured via flow cytometry. E, F Cell migration and invasion were examined via transwell analysis. G Western blot assay was conducted to measure the protein levels of Bax, Bcl-2, MMP2, and MMP9. H–J The inflammatory cytokine secretion (TNF-α, IL-1β, IL-6) was detected via ELISA after transfection. *P < 0.05

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