Fig. 5

CXCL12 is a target of miR-107. A The binding sites of miR-107 and CXCL12 were predicted via DIANA tools. B and C Luciferase activity was measured in chondrocytes co-transfected with CXCL12 3’UTR WT or CXCL12 3’UTR MUT and miR-NC, miR-107 mimic, in-miR-NC or in-miR-107 at 24 h post-transfection. D and E The expression of CXCL12 at mRNA and protein levels was measured in OA and normal samples by qRT-PCR and western blot. F and G The correlation of abundance of CXCL12 and miR-107 or HOTAIR in OA tissues was evaluated by Spearman’s correlation analysis. H The protein level of CXCL12 was measured in chondrocytes transfected with miR-NC or miR-107 mimic by western blot at 24 h post-transfection. I The protein level of CXCL12 was detected in chondrocytes transfected with si-NC or si-HOTAIR by western blot at 24 h post-transfection. n = 3. Data were expressed as mean ± S.D. *P < 0.05