Fig. 4

Deficiency of miR-107 reverses silencing HOTAIR-mediated chondrocyte progression. A Cell proliferation was measured in chondrocytes transfected with si-NC, si-HOTAIR, si-HOTAIR + in-miR-NC or in-miR-107 by CCK-8 at 0, 24, 48 or 72 h. B Cell apoptosis was detected in chondrocytes transfected with si-NC, si-HOTAIR, si-HOTAIR + in-miR-NC or in-miR-107 by flow cytometry at 72 h after culture. C The protein levels of Aggrecan, Collagen II, MMP-13 and MMP-9 were examined in chondrocytes transfected with si-NC, si-HOTAIR, si-HOTAIR + in-miR-NC or in-miR-107 by western blot at 72 h after culture. n = 3. Data were expressed as mean ± S.D. *P < 0.05