Fig. 3

HOTAIR is a decoy of miR-107. A The binding sites of HOTAIR and miR-107 were predicted via DIANA tools. B and C Luciferase activity was measured in chondrocytes co-transfected with HOTAIR WT or HOTAIR MUT and miR-NC, miR-107 mimic, in-miR-NC, or in-miR-107 at 24 h post-transfection. D The levels of HOTAIR and miR-107 were measured after enrichment of Ago2 or IgG RIP for 8 h by qRT-PCR. E The expression of miR-107 was detected in chondrocytes transfected with si-NC, si-HOTAIR, pcDNA or HOTAIR overexpression vector by qRT-PCR at 24 h post-transfection. n = 3. Data were expressed as mean ± S.D. *P < 0.05