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Fig. 7 | Journal of Orthopaedic Surgery and Research

Fig. 7

From: Circ-SPG11 knockdown hampers IL-1β-induced osteoarthritis progression via targeting miR-337-3p/ADAMTS5

Fig. 7

MiR-337-3p targeted ADAMTS5 to participate in OA process. A Western blot was employed to test ADAMTS5 protein expression in CHON-001 cells without treatment or treated with IL-1β and transfected with miR-337-3p, miR-337-3p + ADAMTS5, or negative controls. B CCK8 assay was devoted to determining the viability of CHON-001 cells without treatment or treated with IL-1β and transfected with miR-337-3p, miR-337-3p + ADAMTS5, or negative controls. C Flow cytometry assay was dedicated to monitor the apoptosis rate in CHON-001 cells without treatment or treated with IL-1β and transfected with miR-337-3p, miR-337-3p + ADAMTS5, or negative controls. D The generation of TNF-α and IL-6 was detected by ELISA in CHON-001 cells without treatment or treated with IL-1β and transfected with miR-337-3p, miR-337-3p + ADAMTS5, or negative controls. E ADAMTS5 protein expression was determined by western blot in CHON-001 cells without treatment or treated with IL-1β and transfected with miR-337-3p, miR-337-3p + ADAMTS5 or negative controls. **P < 0.01, ***P < 0.001, ****P < 0.0001.

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