Fig. 6

MiR-337-3p directly targeted ADAMTS5 and negatively regulated ADAMTS5 expression. A Starbase2.0 online database was conducted to predict the potential binding sequences between miR-337-3p and ADAMTS5 3′UTR. B Dual-luciferase reporter system was applied to test the combination between miR-337-3p and ADAMTS5 in OA cells. C RIP assay was devoted to inspecting the combination between miR-337-3p and ADAMTS5 in OA cells. D ADAMTS5 mRNA expression was detected by qRT-PCR in OA tissues and normal tissues. E ADAMTS5 protein expression was measured by western blot in CHON-001 cells treated by 0 ng/mL, 5 ng/mL, 10 ng/mL, 15 ng/mL of IL-1β for 24 h. F Western blot was used to test ADAMTS5 protein expression in CHON-001 cells treated by 10 ng/mL of IL-1β for 0 h, 12 h, 24 h, and 48 h. G Pearson correlation coefficient analyzed the relationship between ADAMTS5 mRNA expression and miR-337-3p expression. H MiR-337-3p expression was detected by qRT-PCR in CHON-001 cells without treatment or treated with IL-1β and transfected with miR-337-3p, anti-miR-337-3p, or negative controls. I ADAMTS5 protein expression was determined by western blot in CHON-001 cells without treatment or treated with IL-1β and transfected with miR-337-3p, anti-miR-337-3p, or negative controls. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001