Fig. 5

MiR-337-3p inhibition allayed si-circ-SPG11-induced promotion in proliferation, repression in apoptosis, inflammatory cytokines secretion, and ECM degradation in OA model cells. A MiR-337-3p expression was measured by qRT-PCR in OA cells without treatment or treated with IL-1β and transfected with si-circ-SPG11, si-circ-SPG11 + anti-miR-337-3p, or negative controls. B The effect of si-circ-SPG11 and anti-miR-337-3p on viability was assessed by CCK8 assay in OA cells without treatment or treated with IL-1β and transfected with si-circ-SPG11, si-circ-SPG11 + anti-miR-337-3p, or negative controls. C The apoptosis rate was measured by flow cytometry in OA cells without treatment or treated with IL-1β and transfected with si-circ-SPG11, si-circ-SPG11 + anti-miR-337-3p, or negative controls. D The role of si-circ-SPG11 and anti-miR-337-3p in inflammatory cytokines secretion was evaluated by ELISA in OA cells treated with or without IL-1β. E Western blot was used to detect the protein expression of MMP13 and Aggrecan in OA cells without treatment or treated with IL-1β and transfected with si-circ-SPG11, si-circ-SPG11 + anti-miR-337-3p, or negative controls. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001