Fig. 3

Circ-SPG11 knockdown reversed IL-1β-induced repression in proliferation, stimulation in apoptosis, inflammatory cytokines secretion, and ECM degradation in CHON-001 cells. A QRT-PCR was employed to test circ-SPG11 expression in CHON-001 cells without treatment or treated with IL-1β and transfected with si-circ-SPG11 or negative control. B CCK8 assay was used to assess the viability in CHON-001 cells without treatment or treated with IL-1β and transfected with si-circ-SPG11 or negative control. C Flow cytometry assay was devoted to measuring the apoptosis in CHON-001 cells without treatment or treated with IL-1β and transfected with si-circ-SPG11 or negative control. D ELISA was conducted to detect the production of TNF-α and IL-6 in CHON-001 cells without treatment or treated with IL-1β and transfected with si-circ-SPG11 or negative control. E Western blot was used to determine the protein expression of MMP13 and Aggrecan in CHON-001 cells without treatment or treated with IL-1β and transfected with si-circ-SPG11 or negative control. **P < 0.01, ***P < 0.001, ****P < 0.0001