Table 1 Primers sequences
From: NDRG1 regulates osteosarcoma cells via mediating the mitochondrial function and CSCs differentiation
Gene | Forward | Reverse |
|---|---|---|
ß-catenin | TGGTGCCCAGGGAGAACCCC | CCCACCCCTCGAGCCCTCTC |
NDRG1 | GGATCAGTTGGCTGAAAT | ATCTTGAGTAGGGTGGTCTT |
Bax | GGAGCTGCAGAGGATGATTG | CCAGTTGAAGTTGCCGTCAC |
Bcl-2 | CTGAGGAGCTTTGTTTCAACCA | TCAAGAAACAAGGTCAAAGGGA |
Fibronectin | GGAGCAAATGGCACCGAGATA | GAGCTGCACATGTCTTGGGAAC |
Vimentin | TGCCGTTGAAGCTGCTAA CTA | CCAGAGGGAGTGAATCCAGAT TA |
VEGF | TTGCCTTGCTGCTCTACCTCCA | GATGGCAGTAGCTGCGCTGATA |
E-cadherin | AATGCCGCCATCGCTTAC | AGTTCGAGGTTCTGGTAGGG |
TCF 4 | TGGCCCTGAGAGGCAGCCAT | GGTCCTCATCGTCATTATTGCTAGAT |
LEF 1 | CCAGCTATTGTAACACCTCA | TTCAGATGTAGGCAGCTGTC |
LRP 5 | CAGCCTGACGCACCCCTTCG | CACCTCCTCGGCTCCTGCCT |
LRP 6 | GCTGGAATGGATGGTTCAAGTCG | CAGAATGGATTTCACGCAGACCC |
Wnt 3a | GTTGGGCCACAGTATTCCTC | ATCCCACCAAACTCGATGTC |
Runx2 | AAGCTTGATGACTCTAAACC | TCTGTAATCTGACTCTGTCC |
Osterix | TGAGGAGGAAGTTCACTATG | CATTAGTGCTTGTTAAAGGGG |
Dlx5 | GCATTACAGAGAAGGTTTCAG | TTTTCACCTGTGTTTGTGTGTC |
Coll I | GCTATGATGAGAAATCAACCG | TCATCTCCATTCTTTCCAGG |
ß-actin | TGGCATTGCCGACAGGATGCAGCAA | CTCCTCATACTCCTGCTTGCTGAT |