Fig. 2

Effects of WNT1 mutation on the mRNA and protein expression levels of BMP2 (osteoblast marker) and RANKL (osteoclast marker) in the MC3T3-E1 cells. The mRNA expression levels of BMP2 (A) and RANKL (B) were determined using quantitative real-time PCR. C The protein expression levels of BMP2 and RANKL were determined using western blotting. D, E Semi-quantitative results of BMP2 and RANKL proteins, respectively, using Image-Pro software. F Osteocalcin expression levels in the osteogenic differentiation medium on day 14 in different groups by ELISA. G Alkaline phosphatase expression levels in different groups by ALP staining. Bars represented 1000 μm * P < 0.05 and ** P < 0.01 indicate significant differences