Fig. 5

Overexpression of GAS2 partially reverses the protective effects miR-520c-3p on IL-1β-stimulated chondrocytes. a Cell viability in control, NC mimic, miR-520c-3p mimic, or miR-520c-3p mimic plus GAS2 overexpression plasmid. b Quantitative analysis of apoptosis rates in each group. c qRT-PCR was performed to assess the caspase-3, Bcl-2, MMP-13, COL2A1, IL-6, and IL-8 in control, IL-1β, IL-1β + miR-520c-3p mimic, and IL-1β + miR-520c-3p mimic +GAS2 groups. d Western blot assay was performed to examine the protein expression levels (cleaved-caspase-3, Bcl-2, MMP-13, COL2A1, IL-6, and IL-8) in chondrocytes transfected with the placebo (control), IL-1β, IL-1β + miR-520c-3p mimic, and IL-1β + miR-520c-3p mimic +GAS2 groups