Skip to main content
Fig. 4 | Journal of Orthopaedic Surgery and Research

Fig. 4

From: CircASH2L facilitates tumor-like biologic behaviours and inflammation of fibroblast-like synoviocytes via miR-129-5p/HIPK2 axis in rheumatoid arthritis

Fig. 4

Knockdown of circASH2L suppresses the tumor-like biologic behaviors of RA-FLSs through miR-129-5p. a-i RA-FLSs were co-transfected with si-circASH2L and/or anti-miR-129-5p. a qRT-PCR analysis of miR-129-5p expression in RA-FLSs. b, c Cell proliferation analysis using MTT assay and Edu assay. d Flow cytometry of cell apoptosis analysis. e Detection of apoptosis-related protein expression using western blot. f Flow cytometry of cell cycle analysis. g, h The migratory and invasive activities analysis of cells using transwell assay. i) The measurement of inflammatory cytokines levels using ELISA. *P<0.05

Back to article page

Journal of Orthopaedic Surgery and Research

ISSN: 1749-799X