Fig. 6

MiR-127-5p exerted its functions in OA chondrocytes by targeting NAMPT. a Overexpression efficiency of NAMPT was determined by western blot assay in OA chondrocytes transfected with NAMPT or vector. b–f OA chondrocytes were transfected with NC, miR-127-5p, miR-127-5p + vector, or miR-127-5p + NAMPT. b Western blot assay was performed to detect the protein expression of NAMPT. c Cell viability was evaluated by CCK-8 assay. d Cell apoptosis was determined using flow cytometry analysis. e The protein levels of Bcl-2, Bax, caspase 3, C-caspase 3, PARP, and C-PARP were analyzed by western blot assay. f Western blot assay was carried out to examine the protein expression of TNF-α, IL-1β, IL-6, MMP3, and collagen type II. *P < 0.05