Fig. 5

NAMPT was a downstream target of miR-127-5p. a The predicted binding sites and its mutant for miR-127-5p on the 3′UTR of NAMPT were shown. b Relative luciferase activity was determined in OA chondrocytes co-transfected with NAMPT-wt or NAMPT-mut and NC or miR-127-5p. c The enrichment of NAMPT was measured by RIP assay in OA chondrocytes transfected with NC or miR-127-5p. d, e The level of miR-127-5p and protein level of NAMPT were determined by qRT-PCR and western blot analyses, respectively, in OA chondrocytes transfected with NC, miR-127-5p, anti-NC, or anti-miR-127-5p. f The protein expression of NAMPT was examined by western blot assay in OA chondrocytes transfected with si-NC, si-circ_0128846#1, si-circ_0128846#1 + anti-NC, or si-circ_0128846#1 + anti-miR-127-5p. g, h NAMPT mRNA and protein levels were measured in OA cartilage tissues and normal cartilage tissues by qRT-PCR and western blot analyses, respectively. i The association between NAMPT mRNA level and miR-127-5p abundance was analyzed in OA cartilage tissues. j The correlation between circ_0128846 level and NAMPT mRNA level was analyzed in OA cartilage tissues. *P < 0.05