Fig. 3

Circ_0128846 directly interacted with miR-127-5p. a The putative binding sites between circ_0128846 and miR-127-5p were predicted by CircInteractome. b Dual-luciferase luciferase reporter assay was used to detect the luciferase activity in OA chondrocytes co-transfected with circ_0128846-wt or circ_0128846-mut and NC or miR-127-5p. c The enrichment of circ_0128846 or miR-127-5p was measured by RIP assay in OA chondrocytes incubated with Anti-Ago2 or Anti-IgG. d, e The expression levels of circ_0128846 and miR-127-5p were examined by qRT-PCR in OA chondrocytes transfected with circ-NC, circ_0128846, si-NC, or si-circ_0128846#1. f The expression of miR-127-5p was analyzed in OA cartilage tissues and normal cartilage tissues by qRT-PCR. g The correlation between circ_0128846 and miR-127-5p expression was analyzed in OA cartilage tissues. *P < 0.05