Skip to main content
Fig. 2 | Journal of Orthopaedic Surgery and Research

Fig. 2

From: Circular RNA circ_0128846 promotes the progression of osteoarthritis by regulating miR-127-5p/NAMPT axis

Fig. 2

Circ_0128846 silence increased cell viability and suppressed apoptosis, inflammation, and ECM degradation in OA chondrocytes. a Knockdown efficiency of circ_0128846 was determined by qRT-PCR in OA chondrocytes transfected with si-NC, si-circ_0128846#1, si-circ_0128846#2, and si-circ_0128846#3. b–e OA chondrocytes were transfected with si-NC or circ_0128846#1. b Cell viability was assessed by CCK-8 assay. c Cell apoptosis was examined using flow cytometry analysis. d Western blot assay was conducted to measure the protein levels of Bcl-2, Bax, caspase 3, C-caspase 3, PARP, and C-PARP. e The protein levels of TNF-α, IL-1β, IL-6, MMP3, and collagen type II were detected by western blot analysis. *P < 0.05

Back to article page