Fig. 8

Mutual effect between miR-578 and PDK1 in cell behaviors and glycolysis of OS cells in vitro. a Western blotting detected relative PDK1 protein expression in 143B and U2OS cells transfected with miR-578, miR-NC, anti-miR-578, or anti-miR-NC. b–k 143B and U2OS cells were transfected with miR-578 comparing to miR-NC-transfected cells, and co-transfected with miR-578 and pcDNA-PDK1 (PDK1) vector paralleled with cells co-transfected with miR-578 and pcDNA. b Western blotting detected relative PDK1 protein expression. c, d MTT assay measured OD value at indicated time-points. e Colony formation assay evaluated number of colonies. f FCM determined apoptosis rate (%). g, h Transwell assay examined numbers of migratory cells and invasive cells. i–k Corresponding kits severally tested glucose consumption, lactate production, and ATP/ADP ratio. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001