Fig. 5

Effect of miR-578 downregulation on role of circ-CNST deficiency in human OS cells in vitro. 143B and U2OS cells were transfected with si-circ-CNST comparing to si-NC-transfected cells, and co-transfected with si-circ-CNST and anti-miR-578 paralleled with cells co-transfected with si-circ-CNST and anti-miR-NC. a RT-qPCR detected relative miR-578 expression. b, c MTT assay measured OD value at indicated time-points. d Colony formation assay evaluated number of colonies. e FCM determined apoptosis rate (%). f, g Transwell assay examined numbers of migratory cells and invasive cells. h–j Corresponding kits severally tested glucose consumption, lactate production, and ATP/ADP ratio. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001