Fig. 2

Role of circ-CNST deficiency in cell behaviors and glycolysis of OS cells in vitro. 143B and U2OS cells were transfected with si-circ-CNST for 24 h comparing to si-NC-transfected cells. a RT-qPCR detected relative circ-CNST expression. b, c MTT assay measured optical density (OD) value at 570 nm at indicated time-points. d Colony formation assay evaluated number of colonies. e FCM determined apoptosis rate (%). f, g Transwell assay examined numbers of migratory cells and invasive cells. h–j Corresponding kits severally tested glucose consumption, lactate production, and ATP/ADP ratio. **P < 0.01, ***P < 0.001, and ****P < 0.0001