Fig. 3

BCL2L12 was a direct target of miR-24-3p. a Alignment of the miR-24-3p seed sequence with the BCL2L12 3′ UTR (CUGAGCC). b The luciferase activity was measured in CHON-001 cells following co-transfecting with WT/MUT BCL2L12 3′-UTR plasmid and miR-24-3p with the luciferase reporter assay. **p < 0.01, compared with miR-NC; Chondrocyte cell line CHON-001 was transfected with miR-24-3p mimics or miR-NC, followed by IL-1β stimulation. The untreated cells were used as control group. c Expression level of BCL2L12 mRNA was determined via reverse transcription quantitative PCR. Data were presented as mean ± SD of three independent experiments. ***p < 0.001, compared with control; ###p < 0.001, compared with IL-1β + miR-NC; d Expression level of BCL2L12 protein in CHON-001 cells was detected with Western blotting