Fig. 5

IRAK3 was a downstream target of miR-591. a The predicted binding sites of miR-591 in the 3′UTR of IRAK3 mRNA were identified by using the starBase database and the relative luciferase activity in chondrocytes co-transfected with wt-IRAK3 or mut-IRAK3 and miR-591 mimic or miRNA NC was determined by dual-luciferase reporter assay. b The level of miR-591 was determined in OA chondrocytes transfected with NC, miR-591, anti-NC, or anti-miR-591. c The level of IRAK3 protein expression level was determined in OA chondrocytes transfected with NC, miR-591, anti-NC, or anti-miR-591. d The IRAK3 mRNA expression in OA cartilage and normal cartilage. e The IRAK3 protein expression in OA cartilage and normal cartilage. *P<0.05