Fig. 3

Circ_SLC39A8 directly interacted with miR-591. a The putative binding sites between circ_SLC39A8 and miR-591 and dual-luciferase luciferase reporter assay were used to detect the luciferase activity in OA chondrocytes co-transfected with circ_SLC39A8-wt or circ_SLC39A8-mut and NC or miR-591. b The expression levels of circ_SLC39A8 in OA chondrocytes transfected with circ-NC, circ_SLC39A8, si-NC, or si-circ_SLC39A8#1. c The expression levels of miR-591 in OA chondrocytes transfected with circ-NC, circ_SLC39A8, si-NC, or si-circ_SLC39A8#1. d The expression of miR-591 was analyzed in OA cartilage tissues and normal cartilage tissues by qRT-PCR. *P<0.05