Fig. 4

WKYMVm peptide decreased the lysosomal activity of mBMSC. mBMSCs incubated with WKYMVm peptide (1 μmol/L) or WKYMVm peptide (1 μmol/L) plus WRW4 peptide (10 μmol/L) for 24 h. a Standard curve drawn according to the manufacturer’s instructions. b Effects of WKYMVm peptide on lysosomal activity (lysosomal activity=(B/ΔT×V)×D. B=amount of 4-methylumbelliferone (4-MU) in sample well calculated using the standard curve (pmol); ΔT=reaction time (min), 30 min in our study; V=sample volume added into the reaction well (ml), 0.02 ml in our study; D=sample dilution factor, 1 in our study). Data represent means ± SD. *P < .05, **P < .01, and ***P < .001 relative to controls in which the concentration of WKYMVm peptide was 0 μmol/L