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Fig. 5 | Journal of Orthopaedic Surgery and Research

Fig. 5

From: Circ-AFF2/miR-650/CNP axis promotes proliferation, inflammatory response, migration, and invasion of rheumatoid arthritis synovial fibroblasts

Fig. 5

The target association of miR-650 and CNP. a The targets of miR-650 were predicted by miRDB, miRwalk, starBase, and Tarbase. b The levels of the predicted targets were detected by qRT-PCR in cells transfected with miR-NC or miR-650 mimic. c CNP and RAC1 protein levels were detected via Western blotting in cells transfected with miR-NC or miR-650 mimic. d The target sites of miR-650 and CNP. e Luciferase activity was measured in cells transfected with WT-CNP 3′UTR or MUT-CNP 3′UTR and miR-NC or miR-650 mimic. f, g CNP level was measured via qRT-PCR and Western blotting in RAFLSs transfected with miR-NC, miR-650 mimic, anti-NC, or anti-miR-650. h, i CNP level was determined by qRT-PCR and Western blotting in RAFLSs with transfection of vector, circ-AFF2 overexpression vector, circ-AFF2 overexpression vector + miR-NC or miR-650 mimic, si-NC, si-circ-AFF2, and si-circ-AFF2 + anti-NC or anti-miR-650. *P < 0.05, ** P< 0.01, *** P< 0.001

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