Fig. 4

The effect of miR-650 knockdown on silencing circ-AFF2-mediated cell proliferation, inflammatory response, apoptosis, migration, invasion, and EMT. RAFLSs were transfected with si-NC, si-circ-AFF2, and si-circ-AFF2 + anti-NC or anti-miR-650. a MiR-650 level was determined by qRT-PCR in the transfected cells. b Cell proliferation was measured using CCK-8 in the transfected cells. c, d IL-6 and IL-10 levels were examined via ELISA. e, f Cell apoptosis was assessed with flow cytometry and caspase3 activity. g Cell migration and invasion were measured through Transwell analysis. h E-cadherin, N-cadherin, and Vimentin levels were determined using Western blotting. **P < 0.01, ***P < 0.001