Fig. 3

The target relationship of circ-AFF2 on miR-650. a The targets of circ-AFF2 were predicted by starBase. b The binding sites of circ-AFF2 and miR-3612 or miR-650. c Luciferase activity was detected in cells transfected with WT-circ-AFF2 or MUT-circ-AFF2 and miR-NC, miR-3612 mimic, or miR-650 mimic. d Circ-AFF2, miR-3612, and miR-650 levels were determined by qRT-PCR after Ago2 or IgG RIP assay. e Circ-AFF2 expression was measured with qRT-PCR after RNA pull-down assay using Bio-miR-NC, Bio-miR-3612, or Bio-miR-650. f MiR-650 expression was detected by qRT-PCR in RAFLSs transfected with miR-NC, miR-650 mimic, anti-NC, or anti-miR-650. g MiR-650 level was measured using qRT-PCR in RAFLSs with transfection of vector, circ-AFF2 overexpression vector, si-NC, or si-circ-AFF2. **P < 0.01, ***P < 0.001