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Fig. 2 | Journal of Orthopaedic Surgery and Research

Fig. 2

From: Circ-AFF2/miR-650/CNP axis promotes proliferation, inflammatory response, migration, and invasion of rheumatoid arthritis synovial fibroblasts

Fig. 2

The effect of circ-AFF2 on cell proliferation, inflammatory response, apoptosis, migration, invasion, and EMT. RAFLSs were transfected with vector, circ-AFF2 overexpression vector, si-NC, or si-circ-AFF2. a Circ-AFF2 expression was measured via qRT-PCR in the transfected cells. b Cell proliferation was detected by CCK-8 in the transfected cells. c, d IL-6 and IL-10 levels were determined with ELISA in the transfected cells. e, f Cell apoptosis was examined via flow cytometry and caspase3 activity. g Cell migration and invasion were detected using Transwell analysis in cells. h E-cadherin, N-cadherin, and Vimentin levels were determined utilizing Western blotting. *P < 0.05, **P < 0.01, ***P < 0.001

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