Skip to main content

Table 1 The forward and reverse primers used for qRT-PCR analysis

From: Silymarin modulates catabolic cytokine expression through Sirt1 and SOX9 in human articular chondrocytes

Gene Primer sequence
iNOS Forward: 5′-CAGCGGGATGACTTTCCAA-3′
Reverse: 5′-AGGCAAGATTTGGACCTGCA-3′
IL-6 Forward: 5′-GCCACTCACCTCTTCAGAACGA-3′
Reverse: 5′-GGCAAGTCTCCTCATTGAATCC-3′
IL-8 Forward: 5′-ATCTGGCAACCCTAGTCTGCTA-3′
Reverse: 5′-CTGTGAGGTAAGATGGTGGCTA-3
IL-10 Forward: 5′-CCCTGTGAAAACAAGAGCAAGG-3′
Reverse: 5′-TCAGTTTCGTATCTTCATTGTC-3
AGCN Forward: 5′-TCGAGGGTGTAGCGTGTAGAGA-3′
Reverse: 5′-GCATCGAGGACAGCGAGG-3′
TIMP-1 Forward: 5′-TGGAAAACTGCAGGATGGACTC-3′
Reverse: 5′-GTTTGCAGGGGATGGATAAACAG-3′
MMP-3 Forward: 5′-CGCATATGAAGTTACTAGCAAG-3
Reverse: 5′-GCATCGATTTTCCTCACGGTTG-3
MMP-9 Forward: 5′-TTGACAGCGACAAGAAGTGG-3′
Reverse: 5′′-TCACGTCGTCCTTATGCAAG-3′
MMP-13 Forward: 5′-TTGACAGCGACAAGAAGTGG-3′
Reverse: 5′-TCACGTCGTCCTTATGCAAG-3′
Sirt1 Forward: 5′-ATGCTTCTAGGCGGACTATGACTT-3′
Reverse: 5′-CACCTTCACCGTTCCAGTTT-3′
GAPDH Forward: 5′-CACTCAGACCCCCACCACAC-3′
Reverse: 5′-GATACATGACAAGGTGCGGCT-3′