Moringa oleifera leaf extracts protect BMSC osteogenic induction following peroxidative damage by activating the PI3K/Akt/Foxo1 pathway

Table 1 The experimental groups and treatments

Group	Medium	Treatment
Control	Osteogenic differentiation medium	No
MO	Osteogenic differentiation medium + 10% Moringa leaf-containing serum	No
OS + H₂O₂	Osteogenic differentiation medium	100 μmol/L H₂O₂
MO + H₂O₂	Osteogenic differentiation medium + 10% Moringa leaf-containing serum	100 μmol/L of H₂O₂
MO + H₂O₂ + Wor	Osteogenic differentiation medium + 10% Moringa leaf-containing serum	100 μmol/L H₂O₂ and 0.5 μmol/L wortmannin
MO + H₂O₂ + DMSO	Osteogenic differentiation medium + 10% Moringa leaf-containing serum	100 μmol/L H₂O₂, 0.5 μmol/L of wortmannin and DMSO (its dosage was the same as the MO + H₂O₂ + Wor group)

OS the third passage of BMSCs were cultured in osteogenic differentiation medium, MO the third passage of BMSCs were cultured in osteogenic differentiation medium + 10% Moringa leaf-containing serum, Wor wortmannin, an inhibitor of PI3K, DMSO solvent of wortmannin

ISSN: 1749-799X