Fig. 4
The effect of H2O2 and Moringa oleifera on expression of osteogenic markers in BMSCs during osteogenic differentiation. a At 7 days after initiation of osteogenic differentiation, mRNA levels of ALP, collagen I, OPN, and Runx2 in BMSCs under the indicated osteogenic induction conditions were measured using qRT-PCR. N = 3 for each group; *P < 0.05 compared with the control group; #P < 0.05 compared with the MO group; @P < 0.05 compared with the OS + H2O2 group; &P < 0.05 compared with the MO + H2O2 group. b Representative images of ALP staining at 7 days and alizarin red staining at 21 days after the initiation of osteogenic differentiation in BMSCs. Magnification, × 40; scale bar, 200 μm