Fig. 3
The effects of H2O2 and Moringa oleifera on expression of Foxo1 and cleaved caspase-3 and pAkt/Akt in BMSCs during osteogenic differentiation. a, b At 48 h after initiation of osteogenic differentiation, protein expression of Foxo1, Akt, and pAkt in BMSCs under the indicated osteogenic induction conditions were measured using western blot assays. a n = 3 for each group; *P < 0.05 compared with the control group; #P < 0.05 compared with the MO group; @P < 0.05 compared with the OS + H2O2 group. b n = 3 for each group; *P < 0.05 compared with the MO + H2O2 + DMSO group, #P < 0.05 compared with the MO + H2O2 group. c Protein expression of cleaved caspase-3 in BMSCs treated with 100 μmol/L H2O2 for the indicated duration was measured using western blot assay. n = 3 for each group; *P < 0.05 compared with the control group. d At 48 h after initiation of osteogenic differentiation, protein expression of cleaved caspase-3 in BMSCs under the indicated osteogenic induction conditions was measured using western blot assays. N = 3 for each group; *P < 0.05 compared with the MO group; #P < 0.05 compared with the OS + H2O2 group; @P < 0.05 compared with the MO + H2O2 group