Fig. 5From: Silencing of Vangl2 attenuates the inflammation promoted by Wnt5a via MAPK and NF-κB pathway in chondrocytesVangl2 silencing can eliminate the effect of Wnt5a on inflammatory activation. a RT-qPCR analysis shows Wnt5a-overexpression efficiency of Wnt5a plasmid in ATDC5. b Effects of Vangl2 and Wnt5a on the expression of Sox9 and Col-2 were measured by Western blotting. c Protein expression of MMP3, MMP9, and MMP13 were also measured by Western blotting. GAPDH was used as a loading control. ns, not statistically significant, *P < 0.05, **P < 0.01; nc/NC, negative control; +, overexpression; -, silencingBack to article page