Fig. 4From: Silencing of Vangl2 attenuates the inflammation promoted by Wnt5a via MAPK and NF-κB pathway in chondrocytesSilencing of Vangl2 prevents Col-2 and aggrecan degradation in OA. a Western blotting of Col-2 in three groups. The images were quantitatively analyzed and normalized to GAPDH. b The change of Col-2 mRNA was quantitated by RT-qPCR. Representative ICC images of Col-2 (c) and the IOD analysis (d). Scale bar, 50 μm. e The aggrecan expression was measured by Western blotting and quantified for three groups. ns, not statistically significant, *P < 0.05, **P < 0.01; IOD, integrated option densityBack to article page