Table 1 Sequence of primers used in ARMS
From: Genetic markers of osteoarthritis: early diagnosis in susceptible Pakistani population
SNPs | Primer | Sequence |
|---|---|---|
TGF β1-29C/T (rs1800470) | T allele | 5′AGGCGTCAGCACCAGTAG3′ |
C allele | 5′ACCACACCAGCCCTGTTC3′ | |
Reverse (common primer) | 5′TAGCAGCAGCAGCAGCA3′ | |
Internal control (reverse) | 5′GCATCTTGCTCTGTGCAGAT3′ | |
Internal control (forward) | 5′TGCCAAGTGGAGCACCCAA3′ | |
IL-6-174G/C (rs1800796) | T Allele | 5′GGATTATGAAGAAGGTAATACTA3′ |
C Allele | 5′CACGAAATTTGAGGATGG3′ | |
Reverse (common primer) | 5′ACAACAGCCCCTCACAGG3′ | |
Internal control (reverse) | 5′CAACTTCATCCACGTTCACC3′ | |
Internal control (forward) | 5′ACACAACTGTGTTCACTAGC3′ | |
CALM1-16C/T (rs12885713) | T allele | 5′GCACCATATATATATCGCGAGGT3′ |
C allele | 5′GCACCATATATATATCGCGAGC3′ | |
Reverse (common primer) | 5′ACTCCCGACCTACCATGGT3′ | |
Internal control (reverse) | 5′GCATCTTGCTCTGTGCAGAT3′ | |
Internal control (forward) | 5′TGCCAAGTGGAGCACCCAA3′ |