Fig. 4

Effect of HSP22-siRNA on the PGF2α-stimulated phosphorylation of p38 MAP kinase in MC3T3-E1 cells. The cells were transfected with 50 nM of HSP22-siRNA (#1) (a), HSP22-siRNA (#2) (b), or negative control-siRNA (Neg) for 24 h and then stimulated by 10 μM of PGF2α for 2 min. The cell lysates were analyzed by Western blot analysis with antibodies against phosphor-specific p38 MAP kinase, p38 MAP kinase, or GAPDH. The histogram shows the quantification data of the phosphorylated levels after normalization with GAPDH levels determined by laser densitometry analysis. The level was plotted as the value of lane 1 to be 1