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Fig. 3 | Journal of Orthopaedic Surgery and Research

Fig. 3

From: HSP22 (HSPB8) positively regulates PGF2α-induced synthesis of interleukin-6 and vascular endothelial growth factor in osteoblasts

Fig. 3

Effect of HSP22-siRNA on the PGF2α-stimulated phosphorylation of p44/p42 MAP kinase in MC3T3-E1 cells. The cells were transfected with 50 nM of HSP22-siRNA (#1) (a), HSP22-siRNA (#2) (b), or negative control-siRNA (Neg) for 24 h and then stimulated by 10 μM of PGF2α or vehicle for 20 min. The cell lysates were analyzed by Western blot analysis with antibodies against phosphor-specific p44/p42 MAP kinase, p44/p42 MAP kinase, or GAPDH. The histogram shows the quantification data of the phosphorylated levels after normalization with GAPDH levels determined by laser densitometry analysis. The level was plotted as the fold increase in comparison with that of the control cells without PGF2α-stimulation

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Journal of Orthopaedic Surgery and Research

ISSN: 1749-799X