Fig. 7

Deficiency of circ_0081001 elevated MTX sensitivity of OS in vivo. U2OS/R cells transfected with sh-NC or sh circ_0081001 were inoculated subcutaneously into the nude mice. The mice were treated with MTX (5 mg/kg MTX) twice a week after injection for 1 week. a, b Tumor volume and weight were examined. c, d The expression of circ_0081001 and miR-494-3p was measured by qRT-PCR in resected tumor tissues. e The protein abundance of TGM2 was detected by WB assay in resected tumor masses. **P < 0.01