Fig. 3

Circ_0081001 directly interacted with miR-494-3p in MTX-resistant OS cells. a The complementary binding sequence of miR-494-3p and circ_00081001 was predicted by circinteractome. b, c Relative luciferase activity was detected in U2OS/R and HOS/R cells after co-transfection with WT-circ_00081001 or MUT-circ_00081001 and miR-494-3p or miR-NC. d The abundance of miR-494-3p was evaluated by qRT-PCR in non-tumor and tumor tissues. e The level of miR-494-3p was examined by qRT-PCR in sensitive tissues and resistant tissues. f The expression of miR-494-3p was measured by qRT-PCR in U2OS, U2OS/R, HOS, and HOS/R cells. g QRT-PCR was conducted to detect the expression of miR-494-3p in U2OS/R and OS/R cells transfected with sh-NC or sh-circ_00081001. **P < 0.01